Figure 2. Expression profile of P. salmonis dot/icm genes during RTS11 and Sf21cell lines kinetic infection.

Gene expression was determined by qRT-PCR, using relative quantification. A: dotB gene expression number; B: icmE gene expression; C: icmK mRNA gene expression; D: dotA gene expression. Gene expression was normalized by the use of ITS like a housekeeping gene. 24 hours post-infection in each cell line was used as calibrator (value = 1).