Table 2. Specific primers for P. salmonis dot/icm genes used for qRT-PCR.
Primer | Sequence | gene |
Ps-DotB-For | 5′-GCT ACA TCT CCA TTT CTT GAC CAT TTC-3 | dotB |
Ps-DotB-Rev3 | 5'- GCA TTA GTG CCG AGC ATT ACA GG-3' | dotB |
PS-IcmK-F | 5′-GCG CCA GAG CAG ATA CAT CAG TAT AAA G-3′ | icmK |
PS-IcmK-R1 | 5′-GCC ACC GGA ACA TCT AAG CCT TTT AA-3′ | icmK |
Ps-IcmE-For1 | 5′-GCC TTG GTT AAG TGT GAC CGT TG-3′ | icmE |
PS-IcmE-Rev1 | 5′-GCT GTC ATT ACC TGC ATT AGA TCA TAG-3′ | icmE |
Ps-DotA-For | 5′-GCT TAT GTC GCC ATT TCT GCA GCA CTT C-3′ | dotA |
Ps-DotA-Rev2 | 5′-CCA CTC ACT CGG CAA ATT AAG CAG-3′ | dotA |