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. 2013 Jan 28;8(1):e54934. doi: 10.1371/journal.pone.0054934

Table 2. Specific primers for P. salmonis dot/icm genes used for qRT-PCR.

Primer Sequence gene
Ps-DotB-For 5′-GCT ACA TCT CCA TTT CTT GAC CAT TTC-3 dotB
Ps-DotB-Rev3 5'- GCA TTA GTG CCG AGC ATT ACA GG-3' dotB
PS-IcmK-F 5′-GCG CCA GAG CAG ATA CAT CAG TAT AAA G-3′ icmK
PS-IcmK-R1 5′-GCC ACC GGA ACA TCT AAG CCT TTT AA-3′ icmK
Ps-IcmE-For1 5′-GCC TTG GTT AAG TGT GAC CGT TG-3′ icmE
PS-IcmE-Rev1 5′-GCT GTC ATT ACC TGC ATT AGA TCA TAG-3′ icmE
Ps-DotA-For 5′-GCT TAT GTC GCC ATT TCT GCA GCA CTT C-3′ dotA
Ps-DotA-Rev2 5′-CCA CTC ACT CGG CAA ATT AAG CAG-3′ dotA