Table 3. Microarray analysis of the transcriptional influence of PA2206 in the presence of hydrogen peroxide.
ORF ID | Gene name | Description | Fold change |
Genes positively regulated by PA2206 | |||
PA0321 | probable acetylpolyamine aminohydrolase | 3.8 | |
PA0323 | spermidine/putrescine-binding periplasmic protein | 2.0 | |
PA0472 | fiuI | probable sigma-70 factor, ECF subfamily | 2.0 |
PA0609 | trpE | anthranilate synthetase component I | 3.0 |
PA0649 | trpG | anthranilate synthase component II | 2.4 |
PA0729.1 | tRNA Glycine | 2.8 | |
PA1409 | aphA | acetylpolyamine aminohydrolase | 6.1 |
PA1410 | periplasmic spermidine/putrescine-binding protein | 4.3 | |
PA1912 | femI | ECF sigma factor, FemI | 2.4 |
PA1961 | probable transcriptional regulator | 2.2 | |
PA2033 | hypothetical protein | 2.2 | |
PA2206 | probable transcriptional regulator | 6.4 | |
PA2214 | major facilitator superfamily (MFS) transporter | 2.2 | |
PA2215 | hypothetical protein | 3.8 | |
PA2216 | hypothetical protein | 2.7 | |
PA2426 | pvdS | sigma factor | 5.3 |
PA2594 | hypothetical protein | 3.3 | |
PA2761 | hypothetical protein | 2.2 | |
PA3229 | hypothetical protein | 3.0 | |
PA3515 | hypothetical protein | 2.0 | |
PA3516 | probable lyase | 2.5 | |
PA3517 | probable lyase | 2.0 | |
PA3530 | hypothetical protein | 2.6 | |
PA4515 | uncharacterized iron-regulated protein | 3.0 | |
PA4516 | hypothetical protein | 2.5 | |
PA4659 | probable transcriptional regulator | 2.2 | |
PA4660 | phr | deoxyribodipyrimidine photolyase | 2.2 |
PA4695 | ilvH | acetolactate synthase isozyme III small subunit | 2.1 |
PA4708 | phuT | haeme-transport protein | 2.0 |
PA4709 | probable hemin degrading factor | 2.5 | |
PA4773 | hypothetical protein | 2.0 | |
PA4881 | hypothetical protein | 7.7 | |
PA5129 | grx | glutaredoxin | 2.2 |
PA5217 | binding protein component of ABC iron transporter | 3.1 | |
Genes negatively regulated by PA2206 | |||
PA0105 | coxB | cytochrome c oxidase, subunit II | 0.37 |
PA0106 | coxA | cytochrome c oxidase, subunit I | 0.37 |
PA0788 | hypothetical protein | 0.36 | |
PA1216 | hypothetical protein | 0.35 | |
PA1217 | probable 2-isopropylmalate synthase | 0.50 | |
PA1317 | cyoA | cytochrome o ubiquinol oxidase subunit II | 0.47 |
PA2166 | hypothetical protein | 0.29 | |
PA2747 | hypothetical protein | 0.42 | |
PA2821 | probable glutathione S-transferase | 0.38 | |
PA3032 | snr1 | cytochrome c Snr1 | 0.29 |
PA3361 | lecB | fucose-binding lectin PA-IIL | 0.51 |
PA3369 | hypothetical protein | 0.36 | |
PA3370 | hypothetical protein | 0.32 | |
PA3371 | hypothetical protein | 0.35 | |
PA3416 | pyruvate dehydrogenase E1 component, β chain | 0.48 | |
PA3417 | pyruvate dehydrogenase E1 component, α subunit | 0.50 | |
PA3451 | hypothetical protein | 0.50 | |
PA3788 | hypothetical protein | 0.40 | |
PA4141 | hypothetical protein | 0.50 | |
PA4738 | conserved hypothetical protein | 0.44 | |
PA4739 | conserved hypothetical protein | 0.48 | |
PA5085 | probable transcriptional regulator | 0.31 | |
PA5481 | hypothetical protein | 0.39 | |
PA5482 | hypothetical protein | 0.37 |
Genes that exhibited a 2-fold or greater alteration in expression in the PA2206C strain compared to the PA2206− mutant strain (p<0.05) are listed. Positive values indicate those genes which were upregulated in the PA2206C strain while values less than 1.0 indicate those genes which were downregulated.