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. 2013 Jan 28;8(1):e53895. doi: 10.1371/journal.pone.0053895

Figure 1. Dose response of 5-aza-2′deoxycytidine and Trichostatin-A induced HERV-Fc1 gag and env mRNA expression.

Figure 1

Effect of different doses of 5-aza-dC (upper row) or TSA (bottom row) on the HERV-Fc1 gag and env extracellular mRNAs expressions. Cells were cultured in medium (untreated control) or in medium containing indicated drug at the indicated concentrations. After 24 h cells were harvested, RNA was extracted and used for HERV-Fc1 gag and env quantification by real-time Q-PCR. Relative changes of HERV-Fc1 gag and env expression in HEK 293, PBMCs and Jurkat cells. Q-PCR results shown are the means of two experiments each performed in triplicates. The results are relative to the untreated control cells. Errors bars represent ± SD. Y-axis relative HERV-Fc1 mRNA expression levels.