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. 2013 Jan 28;8(1):e53895. doi: 10.1371/journal.pone.0053895

Figure 6. Cycloheximide increases endogenous HERV-Fc1 retroviral RNA levels in human cells.

Figure 6

(A) Real-time Q-PCR analyses of the expression of HERV-Fc1 gag extracellular sequences in cells treated with increasing doses of cycloheximide. Graph bars represent relative changes of HERV-Fc1 gag extracellular mRNA. Q-PCR results shown are the means of two experiments each performed in triplicates. Errors bars represent ± SD. Y-axis relative HERV-Fc1 mRNA expression levels. (B) Time response curve of HERV-Fc1 mRNA induction in cells transfected with 5′UTR HERV-Fc1 Gag 6×His expression vector. Graph bars represent relative changes of HERV-Fc1 gag extracellular mRNA. Q-PCR results shown are the means of two experiments each performed in triplicates. Cycloheximide was added 48 h post transfection at the concentration of 10 µg/ml. Errors bars represent ± SD. Y-axis relative HERV-Fc1 mRNA expression levels.