Figure 6.
Rapid washout of a luminally delivered membrane-impermeant inhibitor. After steady-state inhibition, inhibitor concentration in the intestinal lumen was reduced from Co/Kd to 0. (A) Drug concentrations profiles for kinetic (presteady-state) solution of single-crypt diffusion–convection for Co/Kd = 10, Jvo = 10−3 µL/cm2/s, and k−1 = 10−1 min−1. (B) Kinetics of inhibition of fluid secretion after inhibitor washout. Percent inhibition of net fluid secretion as a function of time after washout for Co/Kd = 10, Jvo = 10−3 µL/cm2/s, and indicated k−1. (C) Washout kinetics for the three-dimensional multi-crypt model for same parameters as in A.