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. 2013 Feb;141(2):229–241. doi: 10.1085/jgp.201210870

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© 2013 Gustina and Trudeau

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Proximal domain regulation of steady-state activation may require a covalent link to the channel. (A–F) Channel schematics and two-electrode voltage-clamp recordings of a family of currents from hERG (A), hERG ΔN (B), hERG ΔN plus N1–135 (C), hERG Δeag (D), hERG Δeag plus N1–135 (E), and hERG ΔN plus N1–354 (F). Currents were elicited using the pulse protocol shown. Calibration bars, 1 µA and 200 ms. (G) Steady-state activation (G-V) curves derived from the currents shown in A–F by normalizing the peak outward currents at −50 mV and plotting versus voltage. Data were fit with a Boltzmann function (see Materials and methods) to determine the V_1/2 and k (slope) values. Error bars are the SEM and are within the points if not visible. n ≥ 9 for each construct.