Figure 5.

eag domain regulation of hERG recovery from inactivation. (A) Two-electrode voltage-clamp recordings of tail currents from the constructs indicated, enlarged to show the rising phase at the step to −100 mV. Currents were normalized to the peak for comparison. Calibration bar, 50 ms. (B) Plot of the time constants (τ) of recovery from inactivation derived from single-exponential fits to the rising phase of the tail currents in A from hERG (red triangle), hERG ΔN (open square), hERG ΔN plus N1–135 (closed square), hERG Δeag (open circle), hERG Δeag plus N1–135 (closed circle), and hERG ΔN plus N1–354 (closed diamond). Error bars are the SEM and are within the points if not visible. n ≥ 9 for each construct.