Figure 5.

Effect of extracellular Ca²⁺ removal, nifedipine, CPA, and ryanodine on PMA-induced airway SMC twitching. Line scans from airway wall regions obtained from phase-contrast images (similar to that presented in Fig. 1) showing airway SMC twitching induced by 10 µM PMA (added 30 min before the recordings started) and their sensitivity to: (A) Ca²⁺-free sHBSS, (B) 10 µM nifedipine, (C) 10 µM CPA, or (D) 25 µM ryanodine. PMA-induced SMC twitching was inhibited by removal of extracellular Ca²⁺, nifedipine, CPA, and ryanodine. Each line scan is representative of three experiments in lung slices from two mice.