Figure 2.
Biophysical properties of heterologously expressed human NaV1.9. (A) Voltage dependence of activation time constants (n = 14–17). (B) Voltage dependence of time to peak current (n = 18). Solid line illustrates a representative inward current trace, dashed lines point to the beginning and peak of the current, and the double-headed arrow indicates the time to peak measurement period. (C) Mean whole-cell NaV1.9 currents recorded during 300-ms pulses to −80, −60, −40, −20, and 0 mV (n = 12). Dashed line indicates the zero current level. (D) Voltage dependence of inactivation time constants (n = 13–17). (E) Superimposed steady-state channel availability (open circles) and conductance-voltage relationship (closed circles) for NaV1.9 currents (n = 7 and 16, respectively). Solid lines represent data fitted with Boltzmann functions. (F) Time course of recovery from fast inactivation (holding potential was −120 mV). Line represents data fitted with a two-exponential function (n = 8). Data are represented as means ± standard error of the mean.