Fig. 1.

Immunodetection of CRT during spermiogenesis in C. vulgaris. a Western blot analysis; line 1, SDS-PAGE electrophoretic separation of the antheridial filament cells extract; line 2, molecular mass standards and their weights in kilodaltons; line 3, Western blotting of the antheridial filament cells extract probed with the anti-human CRT antibody; line 4, CRT pre-immune serum; *Proteins revealed b ultrastructure of spermatid at IV stage of spermiogenesis in longitudinal section (immunogold technique). c Spermatid at V stage of spermiogenesis (negative control to immunogold technique). d Ultrastructure of spermatid at V stage of spermiogenesis in longitudinal section (immunogold technique). Arrows show localization of gold grains in particular spermatid compartment. cER, endoplasmic reticulum cisternae; vER, endoplasmic reticulum vesicles; N, nucleus; ne, nuclear envelope; NR, nuclear reticulum; Bar═250 nm