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. 2013 Mar;66:274–289. doi: 10.1016/j.neuropharm.2012.05.025

Fig. 3.

Fig. 3

Distribution of mGlu7a receptors in the amygdala and preferential targeting of large mGlu1α+ ITC neurons. (A) Immunofluorescence micrograph showing intense punctate mGlu7a receptor reactivity in the BLA and in particular around the Imp and IN clusters. Scale bar: 500 μm. (B) This micrograph shows an enlarged view of the IN and the intense labelling around this cluster, but not inside, can be better appreciated. (C) Immunofluorescence micrograph taken at the same focal plane as (B) and showing mGlu1α receptor reactivity in the IN. Scale bar: 125 μm. (D–F) Low and (G–I) high magnification images depicting mGlu1α+ dendrites and spines of large ITC neurons decorated by dense mGlu7a positive terminals. Scale bar: D–I 20 μm. (J) Pre-embedding immunogold/silver labelling for mGlu7a receptors in the BA. Immunometal particles identifying mGlu7a receptors are selectively concentrated in the presynaptic active zone of axon terminals, which establish primarily excitatory synapses with postsynaptic dendrites (solid arrowhead) and spines. Scale bar: 500 nm. Abbreviations: BA, basal amygdala; d, dendrite; Imp, medial paracapsular ITC cluster; IN, main ITC nucleus; LA, lateral amygdala; s, spine.