Table 4.
Efficiency of uptake of fluorescent long dsRNA by eight tick cell lines
| Percentage of cells containing detectable green fluorescence when incubated with dsRNA and: | |||
|---|---|---|---|
| Cell Line | Lipofectamine 2000 | Xtreme | No reagent |
| AVL/CTVM13 | 68 | 73 | 23 |
| BDE/CTVM16 | 55 | 62 | <2 |
| BME/CTVM23 | 54 | 50 | <2 |
| HAE/CTVM9 | 65 | 79 | <2 |
| IRE/CTVM19 | 29 | 62 | <2 |
| IDE8 | 45 | 51 | 4 |
| ISE6 | 53 | 77 | <2 |
| RAE/CTVM1 | 70 | 49 | 18 |
Cells were incubated with fluorescent long dsRNA either alone or with one of two transfection reagents, Lipofectamine 2000 or Xtreme (one tube of cells per treatment)