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. Author manuscript; available in PMC: 2013 Jan 29.
Published in final edited form as: Methods Mol Biol. 2011;725:121–141. doi: 10.1007/978-1-61779-046-1_9

Figure 2. Detection of human Ago2 activity in megakaryocytes.

Figure 2

RISC activity assays were performed using S100 protein extracts (A) or Ago2 immune complex (B) from MEG-01 cell line and incubated in the presence of a 32P-labeled sensor RNA bearing a binding site complementary to human miR-223. (C) Ago2 immune complexes were analyzed by Northern blot for the presence of miR-223. The reactions were analyzed by denaturing PAGE and autoradiography. A 10-nt RNA ladder was used as a size marker (M). Adapted from Landry et al. (35), with permission from The Nature Publishing Group.