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. 2012 Jul 13;445(Pt 3):377–382. doi: 10.1042/BJ20120820

Figure 2. IL-1 pretreatment accelerates down-regulation of CYP2B by NO produced by NOS2.

Figure 2

(A) Hepatocytes (3 days old) were pretreated with 1 mM PB, which was present for the rest of the experiment. After 48 h, the cells were treated with L-NAME (100 μM) and IL-1 (5 ng/ml) for a period of 12 h. The media were removed and the cells were then incubated in media supplemented with either IL-1 plus L-NAME or IL-1 plus 1 mM L-arginine (L-Arg) for the indicated times. Cells were harvested and lysates were prepared for Western blotting. (B) Western blot of CYP2B in cell lysates. (C) Quantitative analysis of the Western blot data and NOx concentrations in the media of the L-arginine-treated cells. Values are means±S.E.M., and CYP2B levels are normalized to GAPDH levels and expressed relative to zero time. *P<0.05 compared with the L-NAME treated group, Student's t test.