Skip to main content
. 2012 Jul 13;445(Pt 3):377–382. doi: 10.1042/BJ20120820

Figure 3. IL-1 pretreatment accelerates down-regulation of CYP2B by the NO donor NOC-18.

Figure 3

(A) Hepatocytes (3 days old) were pretreated with 1 mM PB, which was present for the rest of the experiment. After 48 h, the cells were treated with L-NAME (100 μM) with or without IL-1 (5 ng/ml) for a period of 12 h. Subsequently, NOC-18 (500 μM) was added directly to the media, and cells were harvested 6 or 12 h after NOC-18 addition. (B) Western blot of CYP2B in cell lysates. (C) Quantitative analysis of the data in (B). Values are means±S.E.M. normalized to the GAPDH signals, and are expressed relative to the 6 h L-NAME control group. The numbers within the bars of the NOC-18-treated groups indicate the percentage decreases relative to the respective controls. aP<0.05 compared with control at the same time point; bP<0.05 compared with NOC-18-treated samples without IL-1 pretreatment. One-way ANOVA and Tukey's test. Con, control.