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. Author manuscript; available in PMC: 2013 Jan 29.
Published in final edited form as: Cell Signal. 2011 Aug 17;24(1):77–85. doi: 10.1016/j.cellsig.2011.08.004

Figure 7. LPA induces histone H3 acetylation on the ST2 promoter region.

Figure 7

HBEpCs were treated with LPA (1 μM) for 3 h and then subjected to Chip assay. IgG antibody was used as negative control. ST2 proximal promoter was detected by regular PCR (32 cycles) with primers designed based on human ST2 proximal promoter sequence (accession number: S S74267). Amplified DNA was separated on 1.5 % agarose gel (A). B. After DNA was extracted from chromatin immunoprecipitated complex, levels of ST2 proximal promoter region were determined by Real-time PCR with same primers used in A. Data was normalized to IgG control. Data are the mean ± SD of three values is shown. *p<0.01 versus veh.