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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Neurotoxicology. 2012 Nov 3;34:160–166. doi: 10.1016/j.neuro.2012.10.014

Figure 4. Selective inhibition of TRPC3 reduces the rate of Mn2+-dependent Fura-2 quenching in striatal astrocytes.

Figure 4

(a) Following stimulation with 1 μM ATP, Mn2+ (100 μM) is rapidly taken up by striatal astrocytes, indicated by quenching of Fura-2 fluorescence intensity. (b) Mn-dependent quenching of Fura-2 fluorescence following ATP stimulation is prevented by the TRPC3 inhibitor, Pyr3. (c) 100 μM OAG induces direct influx of Mn2+ from the extracellular medium. (d) Pyr3 inhibits OAG-dependent Mn2+ influx and quenching of Fura-2 fluorescence. Data are presented as mean rate of decay of the Fura-2 signal ± SEM. Vertical bars denote 1 relative fluorescent unit; horizontal bars denote 10 sec (n=20-30 cells per group; * p<0.05, ** p<0.01).