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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Neurochem. 2012 Dec 26;124(4):558–570. doi: 10.1111/jnc.12102

FIGURE 7.

FIGURE 7

Homocysteine-induced secondary p38 MAPK phosphorylation is downstream of ERK MAPK. (A, B) Neuron cultures were treated with 50 μM L-homocysteine (L-Hcy) for 4 hr. 30 min after (post 30′) the onset of homocysteine treatment (A) PD98059 (15 μM) or U0126 (20 μM) and (B) SB203580 (5 μM), SB202190 (7.5 μM) or SB239063 (10 μM) was added and the incubation was continued for the specified time period. Immunoblot analysis was performed using (A) anti-phospho-p38 (upper panel) and anti-p38 (lower panel) antibodies or (B) anti-phospho-ERK (upper panel) and anti-ERK (lower panel) antibodies. Quantification of phosphorylated p38 and ERK MAPK was done by computer-assisted densitometry and Image J analysis. Values are mean ± s.e.m. (n=3). * Significant difference from 0 min (p < 0.001); # significant difference from 4 hr homocysteine treatment (p < 0.001).