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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Mol Cell. 2012 Nov 29;49(2):273–282. doi: 10.1016/j.molcel.2012.10.022

Figure 3. Identification of Potential XIAP and cIAP1 Substrates, Using Respective NEDDylators and SILAC-based Mass Spectrometry.

Figure 3

(A) Workflow for comparing proteins that are NEDDylated with (“Light”) or without (“Heavy”) the NEDDylator.

(B) Distribution of potential XIAP substrate protein Light/Heavy ratios obtained by the SILAC experiments. Each dot represents a protein, from which at least two unique peptides were identified. Light/Heavy ratios of each protein are plotted against the log scale of summed intensity. Known XIAP substrates caspase-7, HtrA2 and Smac are shown in blue; native NEDD8 substrate cullins, including Cul1, 2, 3, 4A, and 5 are shown in red.

(C) Distribution of potential cIAP1 substrate protein Light/Heavy ratios obtained by a similar SILAC experiment. Labels are the same as in (B).

See Figure S3, Table S1 and Table S2.