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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biomaterials. 2012 Dec 28;34(10):2412–2420. doi: 10.1016/j.biomaterials.2012.11.059

Fig 2.

Fig 2

Flow cytometric analysis of purity of isolated granulosa cells (A) retrieved from the first interphase of the discontinuous Percoll gradient (B) and theca cells (C) from the second interphase. Immuno-fluorescent staining for FSHR and CYP19 in granulosa (D–G) and theca cells (H–K); LHR and CYP17A1 in granulosa (L–O) and theca cells (P–S); Negative controls with secondary antibodies only on each cell type (T–W). Images acquired at 100 × magnification. FSHR – follicle-stimulating hormone receptor, LHR – luteinizing hormone receptor, CYP19 – aromatase, CYP17A1 – 17, 20 lyase, IgG – immunoglobulin G, FITC – Fluorescein isothiocyanate, TR – Texas Red.