Fig.4.

PBN reduced infarct volume and improved neurological behavior after tFCI. (a) The brains of vehicle- and PBN- pre treated mice were sliced into 1-mm coronal sections and stained with TTC followed by 4% paraformaldehyde. Infarct volume was significantly reduced by treatment with PBN compared with the vehicle (***p<0.001, n=12, compared with vehicle-treated mice). Using circling and forelimb tests, the PBN- pre treated mice showed significant neurological improvement compared with the vehicle-treated mice after tFCI up to 7 days of reperfusion. The highest score represents the worse neurological outcome (*p<0.05, **p<0.01 and ***p<0.001, n=12, comparison between vehicle-treated mice and PBN-treated mice). Veh indicates vehicle. (b) PBN- post treated mice showed significantly reduced infarct volume and ameliorated neurological behavior (**p<0.01 and ***p<0.001, n=13, compared with vehicle-treated mice). (c) Regional CBF in percent of baseline was assessed by laser Doppler flowmetry before, during, and after tFCI. There were no significant regional CBF differences among vehicle-, PBN 40 mg/kg-, and PBN 60 mg/kg-administrated group (n=3, compared to PBN 0 mg/kg-treated mice).