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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Neurochem. 2012 Dec 28;124(4):523–535. doi: 10.1111/jnc.12111

Fig.5.

Fig.5

Inhibition of C3 increased cell viability and reduces intracellular ROS levels after OGD. (a) Mouse primary cortical neurons were transfected with C3 siRNA and subjected to 3 h of OGD, followed by 6 and 24 h of reperfusion. Cell cytotoxicity was assessed by LDH assay at 490 nm. C3 knock-down neurons showed decreased cell cytotoxicity after OGD (***p<0.001, n=4, comparison between scrambled siRNA and C3 siRNA; -fold to normoxia scrambled). (b) Intracellular ROS levels were measured by DCFH-DA staining. C3 knock-down neurons showed decreased intracellular ROS levels after 3 h of OGD (***p<0.001, n=4, comparison between scrambled siRNA and C3 siRNA; -fold to normoxia scrambled). (c) C3 knock-down neurons also showed down-regulated cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein levels (**p<0.01 and ***p<0.001, n=4, comparison between scrambled siRNA and C3 siRNA; -fold to normoxia scrambled). Nor scr indicates normoxia scrambled.