Figure 3.

Quantification of superoxide production from all cells in the core infarct region detected in situ 3 days after stroke in the ipsilateral cortex (stroke affected; solid bars) compared with the contralateral cortex (nonaffected; open bar) following ischemic stroke in vehicle control and apocynin-treated rats (a). Core infarct regions of the cortex were identified using MCID images generated from unstained forebrain sections adjacent to those analyzed for superoxide, in vehicle control rats (b) and apocynin-treated rats (e). Representative fluorescence micrographs of sections incubated with superoxide-sensitive dihydroethidium in vehicle control ((c), (d)) and apocynin- ((f), (g)) treated rats from the ipsilateral cortex ((d), (g)) and the contralateral mirror image ((c), (f)). Relative fluorescence was quantified by tracing around individual cells and analysed using ImageJ software. Data are presented as mean ± SEM (n = 11 apocynin treated; n = 9 vehicle treated). **P < 0.01 versus contralateral control (ANOVA). Scale bar = 100 μm.