Fig. 4.

PACAP-mediated induction of serine-133 CREB phosphorylation does not require AP firing. (a–c) PACAP induces phosphorylation of CREB at serine-133 in a TTX-insensitive, PKA-dependent manner. Neurons were pre-treated with TTX or H-89 and then treated for 15 min with PACAP. Protein was harvested and subject to western analysis for phospho-CREB (see Methods, normalized in all cases to total CREB, *p < 0.05, n = 4, example blots are shown). (a) and (b) show example westerns and (c) shows quantitation of phospho-CREB levels (normalized to total CREB).