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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 20;1832(3):391–399. doi: 10.1016/j.bbadis.2012.12.005

Figure 4. IGF-1 upregulation of GPX1 is translation dependent.

Figure 4

(A) GPX1 mRNA levels were assessed by quantitative RT-PCR in hAECs exposed to 0–100 ng/mL IGF-1 for 6 hr and 24 hr. No significant difference was detected, n=3. (B) hAECs were exposed to 0–100 ng/mL IGF-1 and cycloheximide (CHX; 10 µg/mL) for 24 hr and GPX1 protein expression was assessed by Western blot analysis. IGF-1 increased GPX1 levels (*P<l0.05 vs. 0 ng/mL IGF-1, #P<0.01 vs. 0 ng/mL IGF-1, n=4), however CHX blocked the IGF-1 effect.