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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Dec 20;1832(3):391–399. doi: 10.1016/j.bbadis.2012.12.005

Figure 5. IGF-1 influences SBP2-GPX1 mRNA complex formation.

Figure 5

(A) Western blot analysis for SBP2 expression in hAECs. Exposure to 0–100 ng/mL IGF-1 for 24 hr did not alter SBP2 levels. (B) After 18 hr of IGF-1 exposure (100 ng/mL), polysome fraction was extracted and subjected to immunoprecipitation using anti-SBP2 monoclonal antibody. Precipitated SBP2 was visualized by Western blot. IGF-1 did not alter immunoprecipitable SBP2 levels. (C) Levels of GPX1 mRNA associated with SBP2 were determined by quantitative RT-PCR in immunoprecipitates using anti-SBP2 monoclonal antibody. *P<0.01, n=8. (D) Levels of GPX4 mRNA associated with SBP2 were determined by quantitative RT-PCR in immunoprecipitates using anti-SBP2 monoclonal antibody. The difference was no statistically significant. n=8.