PKL shares a common ancestor with CHD3-related proteins in animals. The region of CHD proteins spanning from the N-terminal chromodomain to the C-terminus of the D domain were aligned using MAFFT and a phylogenetic tree was generated using neighbor joining. The numbers beneath the branches indicate bootstrap values (percentage of 1000 replications). The Genbank accession number for the protein is listed to the right of the name. CHD proteins were included from A. thaliana (at), B. distachyon (bd), C. elegans (ce), D. melanogaster (dm), D. rerio (dr), H. sapiens (hs), P. patens (pp), P. trichocarpa (pt), and S. moellendorffii (sm). CHD proteins from Arabidopsis are marked with asterisks.