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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Nov 2;1829(2):199–210. doi: 10.1016/j.bbagrm.2012.10.011

Figure 5.

Figure 5

Recombinant PKL-FLAG binds to free DNA and mononucleosomes. (A) Recombinant PKL (44–275 ng) was incubated with 10 ng of 277-bp DNA end-labeled with Alex Fluor 647 and the mixture was analyzed by electrophoresis on a 2% agarose gel. (B) Recombinant PKL (44–275 ng) was incubated with 20 ng of mononucleosomes containing the same end-labeled 277-bp DNA fragment as in panel A and then analyzed by electrophoresis on a 4% PAGE gel.