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. 2013 Feb 15;140(4):719–729. doi: 10.1242/dev.083741

Fig. 4.

Fig. 4.

Wound-induced patterning gene expression is not affected in pbx(RNAi) planarians. (A) Anterior (A) and posterior (P) blastemas of irradiated pbx(RNAi) trunk pieces exhibited normal irradiation-insensitive, wound-induced expression of notum (control, n=5/7; pbx RNAi, n=6/6; transversely amputated), wnt1 (control, n=7/7; pbx RNAi, n=8/8; transversely amputated) and bmp4 (control, n=8/8; pbx RNAi, n=13/13; parasagitally amputated). Cartoon depicts amputation site; thin fragments were used. (B) notum expression (control, n=6/6; pbx RNAi, n=2/6 no expression, n=3/6 reduced expression) in the anterior blastema and wnt1 expression (control, n=6/6; pbx(RNAi), n=0/7 trunk pieces) in the posterior blastema of transversely cut animals 48 hours post-cutting. Black and red arrows indicate normal and reduced/absent expression, respectively. (C) sFRP-1 expression in transversely cut trunk pieces during regeneration; 1, 2 and 4 days (d) shown (pbx RNAi, n=14 trunk pieces for each condition). Scale bars: 200 μm.