Fig. 7.

pbx is required for expression of AP polarity determinants. (A) wnt1 was expressed in cells adjacent to the posterior wound in control regenerating head pieces (12/12) and following pbx(RNAi) (11/11) at 1 dpa. The sustained posterior wnt1 expression observed at 4 dpa in control animals (16/18) was absent following pbx(RNAi) (16/17). wnt11-2 expression was not observed at 1 dpa in control or pbx(RNAi) animals (8/8 and 7/7), became expressed within the posterior blastema of controls by 4 dpa (17/17) and remained absent from pbx(RNAi) animals (17/17). (B) notum was expressed in cells adjacent to the anterior wound within 18 hours of regeneration in control tail pieces (6/6) and became localized to the anterior tip of the blastema by 50 hours (6/6), whereas its expression was not observed at either time point following pbx RNAi (16/16 and 16/16). (C) wnt1 expression was observed adjacent to the anterior wound in control (6/7) and pbx(RNAi) (6/7) regenerating tail pieces at 1 dpa. Anterior wnt1 expression did not persist to 4 dpa in control (6/6) or pbx RNAi (8/8) regenerating tail pieces. Scale bars: 100 μm.