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. 2013 Feb 15;140(4):831–842. doi: 10.1242/dev.088534

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© 2013. Published by The Company of Biologists Ltd

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Fig. 4. — Influence of fat mutations on Fat protein localization and mobility. (A-L) Confocal micrographs of Fat protein staining (green) in wing discs from fat⁸/fat^G-rv flies expressing (A,D) P[acman]V5:fat[68A4], (B,E) P[acman]V5:fatΔEGF[68A4], (C,F) P[acman]V5:fat:FAT4[68A4], (G,J) P[acman]V5:fatΔD[68A4], (H,K) P[acman]V5:fatΔF[68A4] or (I,L) P[acman]V5:fat-mV[68A4]. Upper panels show horizontal sections, lower panels show vertical sections, with E-cadherin staining (red). (M,N) Western blots on wing disc lysates from animals of the indicated genotypes. Upper panel shows Fat antibody staining, lower panel shows a loading control (GAPDH).