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Effect of temperature on retention of heparin lyase-derived disaccharide standards. (A) Room temperature, (B) 40 °C, (C) 50 °C, (D) 60 °C. Extracted ion chromatograms of the dp2 standards are shown. The peaks overlaid on those of the di- and tri-sulfated dp2s correspond to sulfate loss. The disaccharide peaks are: D0A0, ΔHexA-GlcNAc; D2A0, ΔHexA2S-GlcNAc; D0A6, ΔHexA-GlcNAc6S; D2A6, ΔHexA2S-GlcNAc6S; D0S0, ΔHexA-GlcNS; D2S0, ΔHexA2S-GlcNS; D0S6, ΔHexA-GlcNS6S; D2S6, ΔHexA2S-GlcNS6S.
