An example of studying receptor internalization using A
2AAR- selective fluorescent ligand Alexa488-APEC, reproduced from Brand et al. with permission.
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Living CHO cells transiently transfected with adenosine A2A-CFP receptors were observed using SCM 10–60 min post stimulation with Alexa488-APEC.
The bar graph shows a quantification of HEK293 cells expressing a FLAG-A2A receptor showing predominantly cytosolic, membranous and cytosolic, or predominantly membranous receptor labeling. Three hundred cells per condition were manually counted.
Alexa488-binding is abolished in the presence of 10 µM ZM 241,385.
Agonist (2 µM NECA)-induced A2A-CFP receptor internalization is blocked in the presence of hyperosmolaric sucrose.