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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Bioorg Med Chem Lett. 2012 Nov 5;23(1):26–36. doi: 10.1016/j.bmcl.2012.10.112

Figure 2.

Figure 2

An example of studying receptor internalization using A2AAR- selective fluorescent ligand Alexa488-APEC, reproduced from Brand et al. with permission.35
  1. Living CHO cells transiently transfected with adenosine A2A-CFP receptors were observed using SCM 10–60 min post stimulation with Alexa488-APEC.
  2. The bar graph shows a quantification of HEK293 cells expressing a FLAG-A2A receptor showing predominantly cytosolic, membranous and cytosolic, or predominantly membranous receptor labeling. Three hundred cells per condition were manually counted.
  3. Alexa488-binding is abolished in the presence of 10 µM ZM 241,385.
  4. Agonist (2 µM NECA)-induced A2A-CFP receptor internalization is blocked in the presence of hyperosmolaric sucrose.