Maximum-likelihood tree showing phylogenetic relationships between Cygnet River virus isolate 10–01646 (GenBank accession no. JQ693418) and other orthomyxoviruses: Quaranfil virus isolate EG T 377 (accession no. GQ499304), Thogoto virus strain PoTi503 (accession no. AF527530), infectious salmon anemia virus isolate RPC/NB (accession no. AF435424), influenza C virus C/Yamagata/8/96 (accession no. AB064433), influenza B virus B/Wisconsin/01/2010 (accession no. CY115184), and influenza A virus A/California/07/2009(H1N1) (accession no. CY121681). Tree was based on deduced amino acid sequences of the complete matrix protein of orthomyxoviruses, applying 1,000 bootstrap replicates (6). Numbers at nodes indicate percentage of 1,000 bootstrap replicates. Scale bar indicates nucleotide substitutions per site.