Table 2. Nonprimate hepaciviruses primer sequences for 5′UTR, NS3, and NS5B in samples from horses, United Kingdom*.
Primer |
Position |
Sequence, 5′ →3′ |
EQ5→UTROS | Forward outer sense | ACA YYA CCA TGT GTC ACT CCC CCT |
EQ5→UTROAS | Reverse outer antisense | CYC ATG TCC TAT GGT CTA CGA GA |
EQ5→UTRIS | Forward inner sense | ACA CGG AAA YGG GTT AAC CAY ACY C |
EQ5→UTRIAS | Reverse inner antisense | GCC CTC GCA AGC ATC CTA TCA G |
EQNS3OS | Forward outer sense | ATW TGT GAT GAR TGC CAY AGY AC |
EQNS3OAS | Reverse outer antisense | TAG TAG GTB ACA GCR TTA GCY CC |
EQNS3IS | Forward inner sense | TCY AAR GGT GTD AAG CTT GTT GT |
EQNS3IAS | Reverse inner antisense | TGG CAG AAG YTA AGR TGY CTY CC |
EQNS5BIS | Forward outer sense | AAR TGY TTT GAC TCY ACB GTC ACT C |
EQNS5BOIAS | Reverse outer antisense | ACT RTG ACT RAT YGT YTC CCA ACT CG |
EQNS5BIS2 | Forward inner sense | CAY GAT ATA GAH ACT GAG AGR GA |
EQNS5BIAS2 | Reverse inner antisense | TCR TCT TCC TCR ACG CCY TTR CTG G |
*UTR, untranslated region; NS, nonstructural protein.