Table 2. Viral RNA and virus isolation results from ferrets experimentally infected with Nipah virus, Bangladesh strain*.
| Ferret no., sample |
Virus in shedding samples and blood over time, RNA/virus isolation† |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dpi 1 |
Dpi 2 |
Dpi 3 |
Dpi 4 |
Dpi 5 |
Dpi 6 |
Dpi 7 |
Dpi 8 |
Dpi 9 |
Dpi 10 |
|
| B1 | ||||||||||
| NW | – | Indet | +/−‡ | +/+ | ||||||
| OS | – | – | –‡ | +/− | ||||||
| RS | – | Indet | –‡ | +/− | ||||||
| Blood |
– |
|
+/‡ |
|
–‡ |
|
+/§ |
|
|
|
| B2 | ||||||||||
| NW | – | +/− | +/− | +/+ | ||||||
| OS | – | – | +/− | +/− | ||||||
| RS | – | – | – | +/− | ||||||
| Blood |
– |
|
– |
|
+/+ |
|
+/§ |
|
|
|
| B3 | ||||||||||
| NW | – | +/− | –‡ | +/− | ||||||
| OS | – | – | –‡ | +/− | ||||||
| RS | – | – | –‡ | +/+ | ||||||
| Blood |
– |
|
Indet |
|
–‡ |
|
+/§ |
|
|
|
| B4 | ||||||||||
| NW | – | +/− | +/− | +/+‡ | ||||||
| OS | – | – | +/− | +/−‡ | ||||||
| RS | – | – | – | –‡ | ||||||
| Blood |
– |
|
– |
|
– |
|
+/§ |
|
|
|
| B5 | ||||||||||
| NW | – | +/− | +/+ | +/+ | ||||||
| OS | – | +/+ | +/− | +/+ | ||||||
| RS | Indet | – | – | +/− | ||||||
| Blood |
|
Indet |
|
– |
|
+/+ |
|
+/+ |
|
|
| B6 | ||||||||||
| NW | – | – | +/−‡ | NS | +/+ | |||||
| OS | – | – | +/+‡ | NS | +/− | |||||
| RS | – | – | –‡ | NS | +/− | |||||
| Blood |
|
– |
|
– |
|
+/+‡ |
|
NS |
+/− |
|
| B7 | ||||||||||
| NW | – | +/− | +/+ | +/+‡ | ||||||
| OS | – | +/+ | +/+ | +/−‡ | ||||||
| RS | – | – | – | +/+‡ | ||||||
| Blood |
|
+/+ |
|
– |
|
+/− |
|
+/+‡ |
|
|
| B8 | ||||||||||
| NW | – | – | –‡ | +/+ | ||||||
| OS | – | – | +/−‡ | +/− | ||||||
| RS | – | – | –‡ | +/+ | ||||||
| Blood | – | – | +/+‡ | +/+ | ||||||
*Dpi, days postinfection; NW, nasal wash; –, Nipah virus not detected by reverse transcription PCR (RT-PCR) for N gene; therefore, virus isolation was not attempted for this sample; empty cells represent days on which sampling was not scheduled for that animal; Indet, mean cycle threshold value >39.1 (the defined cutoff value for positive samples); +/−, virus detected by RT-PCR but negative by virus isolation; +/+, sample positive by RT-PCR and virus isolation; OS, oral sample; RS, rectal sample; NS, not sampled. †RNA detection by RT-PCR. ‡Pyrexia first detected. §Virus isolation not attempted for this sample.