Table 1. Extension rates, mutation frequencies, and stalling factors after matches and different mis-matches in a DNA systema.
Reactants | kG (h-1) | ktotal (h-1) | fC/fT/fA | Fidelity (fG) | SG |
---|---|---|---|---|---|
1tg, 2a, 3a-t | 0.06 | 0.18 | 0.15/0.09/0.35 | 0.41 | 14 |
1tg, 2c, 3a-t | 0.86 | 1.3 | 0.03/0.03/0.07 | 0.87 | - |
1tg, 2g, 3a-t | 0.09 | 0.46 | 0.04/0.02/0.81 | 0.13 | 9.3 |
1tg, 2t, 3a-t | 0.1 | 0.2 | 0.07/0.06/0.23 | 0.63 | 8.4 |
1tc, 2a, 3a-t | 0.06 | 0.19 | 0.12/0.09/0.45 | 0.34 | 20 |
1tc, 2c, 3a-t | 0.01 | 0.04 | 0.14/0.12/0.48 | 0.26 | 110 |
1tc, 2g, 3a-t | 1.2 | 1.8 | 0.01/0.01/0.1 | 0.88 | - |
1tc, 2t, 3a-t | 0.02 | 0.06 | 0.1/0.12/0.47 | 0.31 | 74 |
1ta, 2a, 3a-t | 0.04 | 0.13 | 0.11/0.11/0.5 | 0.28 | 13 |
1ta, 2c, 3a-t | 0.02 | 0.08 | 0.1/0.14/0.54 | 0.22 | 27 |
1ta, 2g, 3a-t | 0.05 | 0.19 | 0.09/0.09/0.59 | 0.24 | 9.4 |
1ta, 2t, 3a-t | 0.48 | 0.64 | 0.01/0.01/0.07 | 0.91 | - |
Underlined reactants indicate a matched base pair. Reactions were analyzed by MS. See Supporting Table S6 and Figures S7-9 for more details.