Table 2.
Extension rates, mutation frequencies, and stalling factors after a single mis-match for RNA vs. DNA templates.b
| Reactants | kG (h-1) | ktotal (h-1) | k’G (h-1M-1) | fC/fT/fA | Fidelity (fG) | SG |
|---|---|---|---|---|---|---|
| 5uu, 2a, 4a-t | 5.2 | 5.4 | 1400 | 0/0/0.01 | 0.99 | - |
| 5uu, 2c, 4a-t | 0.22 | 0.66 | 61 | 0.1/0.1/0.59 | 0.21 | 24 |
| 5uu, 2g, 4a-t | 1.3 | 1.9 | 370 | 0.01/0.01/0.19 | 0.79 | 4 |
| 5uu, 2t, 4a-t | 0.09 | 0.26 | 25 | 0.08/0.12/0.41 | 0.39 | 58 |
| 1tt, 2a, 4a-t | 1.9 | 1.9 | 510 | 0/0/0.01 | 0.99 | - |
| 1tt, 2c, 4a-t | 0.04 | 0.14 | 9 | 0.1/0.12/0.53 | 0.25 | 52 |
| 1tt, 2g, 4a-t | 0.12 | 0.32 | 34 | 0.01/0.02/0.66 | 0.3 | 15 |
| 1tt, 2t, 4a-t | 0.06 | 0.16 | 17 | 0.02/0.02/0.62 | 0.34 | 30 |
| 5uu, 2a, 3a-t | 0.65 | 0.95 | 180 | 0.01/0.03/0.11 | 0.85 | - |
| 5uu, 2c, 3a-t | 0.02 | 0.08 | 5 | 0.08/0.06/0.66 | 0.2 | 34 |
| 5uu, 2g, 3a-t | 0.2 | 0.64 | 57 | 0.04/0.09/0.55 | 0.32 | 3.2 |
| 5uu, 2t, 3a-t | 0.03 | 0.14 | 9 | 0.07/0.16/0.55 | 0.22 | 19 |
| 1tt, 2a, 3a-t | 2.5 | 2.5 | 680 | 0/0/0.01 | 0.99 | - |
| 1tt, 2c, 3a-t | 0.03 | 0.09 | 9 | 0.06/0.06/0.57 | 0.32 | 79 |
| 1tt, 2g, 3a-t | 0.15 | 0.8 | 43 | 0.01/0.01/0.9 | 0.08 | 16 |
| 1tt, 2t, 3a-t | 0.15 | 0.4 | 43 | 0.03/0.07/0.43 | 0.47 | 16 |
b
Second-order rate constants are labeled k’. Rates measured by MS assay; see Supporting Table S7 and Figures S3-6 for more details.