Table 5.
Rate of template-free extension.d
| Monomer | Conversion after 96 h | Product distribution (%) dimer (12) : free phosphate (13) : pyrophosphate (14) | k′ (h-1M-1) |
|---|---|---|---|
| dAMP-OAt (3a) | 99 % | 97 : 1 : 2 | 3.0 |
| dCMP-OAt (3c) | 99 % | 94 : 5 : 1 | 3.2 |
| dAMP-ImH+ (4a) | 84 % | 84 : 14 : 2 | 0.5 |
| dCMP-ImH+ (4c) | 75 % | 82 : 16 : 2 | 0.4 |
d
Second-order rate constant for conversion (k′) is given, together with the extent to which hydrolysis product (dNMP-OH) and symmetrical pyrophosphate were formed at the end of the assay. See Scheme 4 for structures.