Table 2. Ct values of Schmallenberg virus–positive Culicoides spp. biting midges collected August–September 2011, the Netherlands*.
| Pool no. | Pooled heads |
Individual abdomens |
|||||
|---|---|---|---|---|---|---|---|
| Species identification by morphologic examination | Ct value |
Species identification by DNA sequencing | Ct value |
||||
| First test | Second test | First test | Second test | ||||
| 95-D | C. chiopterus | 27.88 | NA | C. chiopterus | 24.59 | NA | |
| 501 | C. chiopterus | 35.36 | 34.98 | C. chiopterus | 36.45 | 35.07 | |
| 9-A | C. obsoletus complex | 30.44 | NA | C. scoticus | 24.75 | NA | |
| 18-C | C. obsoletus complex | 28.24 | NA | C. scoticus | 24.95 | NA | |
| 32-B | C. obsoletus complex | 21.84 | NA | C. scoticus | 18.32 | NA | |
| 259 | C. obsoletus complex | 19.60 | NA | C. scoticus | 18.16 | NA | |
| 275 | C. obsoletus complex | 20.72 | NA | C. scoticus | 20.39 | NA | |
| 276 | C. obsoletus complex | 36.02 | 36.78 | C. scoticus | 36.68 | NA | |
| 293 | C. obsoletus complex | 20.43 | NA | No reliable sequence | 19.95 | NA | |
| 294 | C. obsoletus complex | 24.60 | NA | C. obsoletus sensu stricto | 20.06 | NA | |
| 368 | C. obsoletus complex | 25.21 | NA | C. scoticus | 21.80 | NA | |
| 385 | C. obsoletus complex | 20.67 | NA | C. scoticus | 20.25 | NA | |
| 405 | C. obsoletus complex | 23.38 | NA | C. scoticus | 21.64 | NA | |
| 434† | C. obsoletus complex | 23.68 | NA | C. scoticus | 23.10 | NA | |
*Ct values determined by reverse transcription PCR. All midges were collected from cattle in the Ermelo municipality except no. 95-D, which was collected from sheep in the Midden-Drente municipality. Ct, cycle threshold; NA, not applicable. †Two abdomens from this pool were positive; Ct values for the second abdomen were 35.75 and 35.37.