Table 3.
Determination of the seroconvertion time of HBV infection by the designed ELISA assay using two BBI standard seroconvertion panels
| Seroconvertion panels | HBs Ag subtype | Cocentration (ng/ml) | Post infection (Day) | Designed assay (s/c) | Commercial kit (s/c) |
|---|---|---|---|---|---|
| PHM 912-01 | ad | <0.01 | 0 | 0.8 | 0.7 |
| 2 | ad | <0.1 | 5 | 0.9 | 1 |
| 3 | ad | <0.1 | 7 | 0.8 | 0.9 |
| 4 | ad | <0.1 | 12 | 0.8 | 0.6 |
| 5 | ad | <0.1 | 17 | 0.8 | 0.5 |
| 6 | ad | <0.1 | 20 | 0.8 | 0.4 |
| 7 | ad | 0.1 | 24 | 0.8 | 0.5 |
| 8 | ad | >2.6 | 42 | 1.5 | 3.6 |
| 9 | ad | >2.6 | 47 | 2.5 | 10.7 |
| PHM 916-01 | ay | <0.1 | 0 | 0.6 | 0.6 |
| 2 | ay | <0.1 | 7 | 0.8 | 0.7 |
| 3 | ay | <0.1 | 41 | 0.9 | 0.6 |
| 4 | ay | <0.1 | 43 | 0.8 | 0.8 |
| 5 | ay | <0.1 | 48 | 0.8 | 0.8 |
| 6 | ay | <0.1 | 50 | 0.8 | 0.7 |
| 7 | ay | <0.1 | 55 | 0.8 | 0.8 |
| 8 | ay | <0.1 | 57 | 0.8 | 0.8 |
| 9 | ay | 0.2 | 62 | 0.8 | 0.7 |
| 10 | ay | 0.5 | 65 | 1.2 | 1.2 |
| 11 | ay | 2.3 | 69 | 1.34 | 2.2 |
Samples with signal-to cutoff (s/c)>1.0 were considered reactive