Table 4.

Reactivity of the designed ELISA assay with different panels of serum samples

Samples	Designed assay		Commercial kit
	Positive	Negative	Positive	Negative
Positive panel (n=198)	196	2	198	0
Negative panel (n=250)	1	249	0	250
Unselected panel (n=100)	7	93	7	93

Unselected panel refers to serum samples collected from different referral hepatitis diagnostic laboratories without prior information about their hepatitis B status. Positive and negative panels were collected from the Iranian Blood Transfusion Organization based on reactivity with the commercial ELISA kit which was employed as a gold standard