Table 1.

List of used primers and PCR conditions

Primer name	Sequence	Enzyme site	Fragment length	PCR conditions
F-COL1A2	5′GGTACCCAGTATGATGGAAAAGGAGTTGG3′	KpnI	3120	Annealing: 60°C
R-COL1A2	5′AGATCTAGCCCTGTAGAAGTCTCCATC3′	BglII		Extension: 6 min 10 s
F-COL1A1	5′GAATTCCAGCTGTCTTATGGCTATG3′	EcoRI	3183	Annealing: 68°C
R-COL1A1	5′AGATCTAGCCCGGTAGTAGCG3′	BglII		Extension: 6 min 20 s
F-Amp	5′AGATCTTATATATGAGTAAACTTGGTCTGACAG3′	BglII	923	Annealing: 45°C
R-Amp	5′AGATCTAATAATATTGAAAAAGGAAGAGTATG3′	BglII		Extension: 120 s
F-Oligo	5′TCGAGAAAAGAGAGGCTGAAGCTGGTACCTTT3′	XhoI
R-Oligo	5′CTAGAAAGGTACCAGCTTCAGCCTCTCTTTTC3′	XbaI
F-Backbone	5'AATCTTAAGCACGTCCGACGGCGGCCC 3'	AflII	4120	Annealing: 68°C
R-Backbone	5'ATTAGCTAGCGGTTTAGTTCCTCACCTTGTCGTATTATACTATGC3'	NheI		Extension: 4 min 20 s
F-Hygro	5'ATTAGCTAGCATGAAAAAGCCTGAACTCA 3'	NheI	1126	Annealing: 56°C
R-Hygro	5'ATACTTAAGCTATTCCTTTGCCCTCG 3'	AflII		Extension: 65 s