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Screening of recombinant clones containing GRA5 gene. GRA5 PCR product was inserted into pcDNA3.1 plasmid and the recombinant plasmid, pGRA5, was transformed into DH5α bacteria. Screening of recombinant clones was performed by PstI restriction digestion. Digestion of recombinant plasmid harboring GRA5 gene resulted in two DNA fragments of 4243 and 1626 bp
