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. 2011 Jul-Sep;3(3):135–141.

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Copyright © 2011 Avicenna Research Institute

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Western blot analysis of expression of GRA5 in HEK 293-T cell. Expression of the GRA5 protein was evaluated in HEK 293-T cells transfected with pGRA5. The recombinant protein was probed with the mAb anti-GRA5 TG17-113 and GRA5 was found as a band of about 18 kDa, which was about the same size of the native protein (Lanes 2 and 3). Untransfected HEK 293-T cells were used as the negative control (Lane 1) and takyzoites of RH strain were used as the positive control (Lane 4)