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Agarose gel (1%w/v) electrophoresis. A) Isolation of ctxB by PCR. Lane 1 and 2: expected single bands of ctxB; Lane M: DNA size marker (Fermentase, Cat No: SM 1113). B) Digestion of recombinant vector by restriction endonucleases after plasmid extraction. Lane 1: recombinant vector, pQE30-ctxB, digested with BamHI and Hind III; Lane 2: pQE30 digested with the same enzymes. Lane M: DNA size marker (Fermentase, Cat No: SM 1113)
