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Immunoblot analysis of 3F2D8 monoclonal antibody reactivity with human IgG subclasses. Lanes 1 to 4 represent reduced forms of IgG1, IgG2, IgG3 and IgG4 paraproteins, respectively. Lanes 5 to 7 represent non reduced forms of whole molecule, Fab and Fc fragments of IgG1. Lanes 8 to 10 represent non reduced forms of IgG2, IgG3 and IgG4 paraproteins, respectively
