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. 2012 Dec 26;12:304. doi: 10.1186/1471-2180-12-304

Table 1.

Characterization of the low-virulence L. monocytogenes strains

Strains Sub-cutaneous test
 Phenotypic Groupc Mutations Genotypic Groupd MLST
 PFGE types
Mean (log spleens) ± S.D.a I/Tb Sequence types ApaI AscI
CHU 860776 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2
 2
CNL 895803 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2
 2
CNL 895804 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2
 2
CNL 895806 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2
 2
CNL 895809 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2a
 2
CNL 895793 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 2a
 2
SO49 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 3
 23
AF10 e
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 3
 23
99EB24LM
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 3
 23
99EB04LM
 0
 0/5
 I
 PrfA K220T, truncated InlA (76 AA)
 Ia
 13
 3
 23
BO18 e
 1.31
 1/5
 I
 PrfAΔ174-237, truncated InlA (188 AA)
 Ib
 31
 77a
 61b
BO38 e
 0
 0/5
 I
 PrfAΔ174-237, truncated InlA (188 AA)
 Ib
 31
 77a
 61b
AF95 e
 0
 0/5
 I
 PrfAΔ174-237, truncated InlA (188 AA)
 Ib
 31
 77a
 61c
99EB15LM
 0
 0/5
 I
 PrfAΔ174-237, truncated InlA (188 AA)
 Ib
 31
 21a
 20
NP 26
 0
 0/5
 I
 PrfA K130Q
 Ic
 2
 61a
 3
454 e
 3.26 ± 0.53
 3/20
 II
 mutated PC-PLC (D61E, L183F, Q126K, A223V)
  
 10
 9
 11
CNL 895807 e
 3
 1/25
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 1
 1
416 e
 0
 0/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 1
 1
417 e
 2.81 ± 1.47
 2/20
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 1
 1
BO43 e
 2.53
 1/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 1a
 1a
CNL 895795 e
 0
 0/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 1a
 1a
DSS794AA1
 0
 0/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 144
 33a
DSS1130BFA2
 0.47
 1/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 143
 129
DPF234HG2
 2.76 ± 0.04
 2/5
 III
 truncated InlA (25 AA), mutated InlB (A117T, V132I), PI-PLC T262A
 IIIa
 193
 145
 33b
AF105 e
 0
 0/5
 III
 truncated InlA (576 AA)
 IIIb
 9
 81
 64
442 e
 0
 0/5
 IV
  
  
 1
 6
 7
02-99 SLQ 10c Al
 2.9 ± 0.05
 2/5
 IV
  
  
 1
 11
 7
3876
 3.42 ± 0.2
 3/5
 IV
  
  
 1
 142
 113
3877
 2.7 ± 0.2
 3/5
 IV
  
  
 1
 142
 113
N2
 3.59 ± 0.48
 2/5
 IV
  
  
 10
 11
 4b
CR282 e
 3.01 ± 0.61
 2/10
 IV
  
  
 195
 158
 85
LSEA 99–23 f
 4.49 ± 0.89
 3/5
 IV
 truncated InlA (576 AA)
  
 9
 21a
 20
LSEA 99-4f
 3.67 ± 0.81
 3/5
 IV
  
  
 198
 48
 101
09-98 SRV 10a Al1
 0
 0/5
 IV
  
  
 4
 37
 38b
449 e
 0
 0/5
 V
 3 AA deletion at position 742 in InlA
  
 194
 8
 6
BO34 e
 3.63 ± 0.56
 5/10
 V
  
  
 2
 4a
 3
464 e
 2.59 ± 0.39
 9/15
 V
  
  
 1
 9c
 4a
09-98 SRV 10b Al2
 3.54 ± 0.27
 3/5
 V
  
  
 54
 135
 124
11-99 SRV 1a Al
 0
 0/5
 V
  
  
 4
 37
 38b
09-98 HPR 50a Al1
 0
 0/5
 V
 3 AA deletion at position 742 in InlA
  
 6
 67a
 98a
436 e
 2.81 ± 0.68
 12/20
 VI
  
  
 2
 4
 3
LSEA 00–14 f
 0
 0/5
 VI
  
  
 2
 106
 3a
04-99 EBS 1 lb Al 2.53 ± 1.76 2/5 VI     54 139 125

a Log numbers of Listeria recovered from spleens three days after sub-cutaneous injection into the left hind footpads of immunocompetent Swiss mice with 104 CFU in 50 μL. Values are from infected mice.

b Ratio of infected mice to inoculated mice in sub-cutaneous test.

c These groups are based on combined values of cell invasion, plaque formation, and phospholipase activities.

d These groups are based on sequencing of prfA, plcA, plcB, inlA and inlB genes.

e Results already published in Roche et al. [7].

f Results already published in Kerouanton et al. [10].