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Summary of purification of laccase secreted by P. pastoris pPICZαB lcc-5
| Purification Steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification fold |
|---|---|---|---|---|---|
| Crude extract |
370 |
18 |
20.5 |
100 |
1 |
| Ammonium sulfate precipitation |
138.24 |
3.6 |
38.4 |
37.3 |
~1.9 |
| Sephadex G75 + SuperdexG200 | 90 | 1.06 | 84.9 | 24.3 | ~4.0 |
